ca.cioos b4844ae2-d9c1-42f2-ae4d-a8f994c395a0 University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X Hakai Institute Campbell River Canada data@hakai.org https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Hakai Institute Campbell River Canada data@hakai.org https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Hakai Institute Campbell River Canada data@hakai.org https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Tula Foundation Vancouver Canada https://tula.org WWW:LINK https://ror.org/0029jxk29 Department of Fisheries and Oceans Ottawa Canada https://www.dfo-mpo.gc.ca WWW:LINK https://ror.org/02qa1x782 2026-09-09T21:35:45.680Z revision 2026-09-09 publication ISO 19115-1 Geographic information - Metadata First Edition 2014-04-01 ISO 19115-2 Geographic information - Metadata Second Edition 2019-01 ISO 19115-1 Geographic Information - Metadata Amendment 1 2018-02 Canadian Integrated Ocean Observing System Metadata Profile of ISO 19115 2019-11-06 publication Putting fjord biodiversity on the map for Britsh Columbia conservation planning Mettre la biodiversité des fjords sur la carte pour la planification de la conservation en Colombie-Britannique 2023-01-31 creation 2026-05-17 publication 1.0 https://doi.org/10.21966/n141-m959 University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Novotny, Andreas andreas.novotny@su.se https://orcid.org/0000-0001-8910-6183 University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Novotny, Andreas andreas.novotny@su.se https://orcid.org/0000-0001-8910-6183 University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Novotny, Andreas andreas.novotny@su.se https://orcid.org/0000-0001-8910-6183 University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Novotny, Andreas andreas.novotny@su.se https://orcid.org/0000-0001-8910-6183 University of Otago Dunedin New Zealand http://www.otago.ac.nz/ WWW:LINK https://ror.org/01jmxt844 Adams, Clare clare.im.adams@gmail.com https://orcid.org/0000-0002-3379-7795 University of Otago Dunedin New Zealand http://www.otago.ac.nz/ WWW:LINK https://ror.org/01jmxt844 Adams, Clare clare.im.adams@gmail.com https://orcid.org/0000-0002-3379-7795 University of Otago Dunedin New Zealand http://www.otago.ac.nz/ WWW:LINK https://ror.org/01jmxt844 Adams, Clare clare.im.adams@gmail.com https://orcid.org/0000-0002-3379-7795 Hakai Institute Campbell River Canada https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Clemente-Carvalho, Rute https://orcid.org/0000-0001-6922-9418 Hakai Institute Campbell River Canada https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Clemente-Carvalho, Rute https://orcid.org/0000-0001-6922-9418 Hakai Institute Campbell River Canada https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Clemente-Carvalho, Rute https://orcid.org/0000-0001-6922-9418 Hakai Institute Campbell River Canada https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Clemente-Carvalho, Rute https://orcid.org/0000-0001-6922-9418 Hakai Institute Campbell River Canada https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Jordison, Drew drew.jordison@hakai.org https://orcid.org/0009-0008-0197-9261 Hakai Institute Campbell River Canada https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Jordison, Drew drew.jordison@hakai.org https://orcid.org/0009-0008-0197-9261 Hakai Institute Campbell River Canada https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Jordison, Drew drew.jordison@hakai.org https://orcid.org/0009-0008-0197-9261 Hakai Institute Campbell River Canada https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Jordison, Drew drew.jordison@hakai.org https://orcid.org/0009-0008-0197-9261 Hakai Institute Campbell River Canada https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Jordison, Drew drew.jordison@hakai.org https://orcid.org/0009-0008-0197-9261 Hakai Institute Campbell River Canada https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Kellogg, Colleen colleen.kellogg@hakai.org https://orcid.org/0000-0003-4048-5316 Hakai Institute Campbell River Canada https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Kellogg, Colleen colleen.kellogg@hakai.org https://orcid.org/0000-0003-4048-5316 Hakai Institute Campbell River Canada https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Kellogg, Colleen colleen.kellogg@hakai.org https://orcid.org/0000-0003-4048-5316 Hakai Institute Campbell River Canada https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Kellogg, Colleen colleen.kellogg@hakai.org https://orcid.org/0000-0003-4048-5316 Hakai Institute Campbell River Canada https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Lemay, Matthew matt.lemay@hakai.org https://orcid.org/0000-0001-7051-0020 Hakai Institute Campbell River Canada https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Lemay, Matthew matt.lemay@hakai.org https://orcid.org/0000-0001-7051-0020 Hakai Institute Campbell River Canada https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Lemay, Matthew matt.lemay@hakai.org https://orcid.org/0000-0001-7051-0020 Hakai Institute Campbell River Canada https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Lemay, Matthew matt.lemay@hakai.org https://orcid.org/0000-0001-7051-0020 Hakai Institute Campbell River Canada https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 Lemay, Matthew matt.lemay@hakai.org https://orcid.org/0000-0001-7051-0020 University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Hunt, Brian b.hunt@oceans.ubc.ca https://orcid.org/0000-0003-4718-4962 University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Hunt, Brian b.hunt@oceans.ubc.ca https://orcid.org/0000-0003-4718-4962 University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Hunt, Brian b.hunt@oceans.ubc.ca https://orcid.org/0000-0003-4718-4962 University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Hunt, Brian b.hunt@oceans.ubc.ca https://orcid.org/0000-0003-4718-4962 University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Hunt, Brian b.hunt@oceans.ubc.ca https://orcid.org/0000-0003-4718-4962 University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Hunt, Brian b.hunt@oceans.ubc.ca https://orcid.org/0000-0003-4718-4962 University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Hunt, Brian b.hunt@oceans.ubc.ca https://orcid.org/0000-0003-4718-4962 Fjords are deep-water estuaries that support a wide range of marine habitats for pelagic fish. Yet, knowledge gaps in fish distribution and habitat use limit the implementation of efficient marine conservation strategies within fjords. Using British Columbia fjords as a case study, we demonstrated that eDNA metabarcoding combined with species traits can enhance the detection and monitoring of conservation priority species (CPS) within systematic conservation planning frameworks. Samples were collected at four time-points: late January/February (winter), May (spring), August (summer), and November (fall) 2023 from the five British Columbia Fjords: Indian Arm (səl ̓ilwət in hən̓q̓əmin̓əm̓), Howe Sound (Átl'ḵa7tsem in Squamish), Toba Inlet (yɛkʷamɛn in Homalco), Bute Inlet, and Rivers Inlet (Wánuǧvaxsiwa in Oowekyala). Three to five stations were sampled within each fjord and discrete 2 L environmental DNA (eDNA) samples were collected using Niskin bottles at the surface, 10 m below the surface, 100 m below the surface, and 10 m above the bottom. From the eDNA samples, the fish community was mapped using metabarcoding targeting a small region of the mitochondrial 12S rRNA gene using MiFish-U universal primer set. Independent sequencing libraries were prepared for each of the four seasonal datasets, and each was sequenced using Illumina MiSeq V3 chemistry at the Hakai Genome Lab on Quadra Island, BC. Spatiotemporal eDNA patterns confirmed the presence of endangered eulachon (Thaleichthys pacificus) across three fjords and identified deep fjord habitats as conservation priorities for mesopelagic species, including sensitive elasmobranchs and diel vertical migrators such as northern lampfish (Stenobrachius leucopsarus). eDNA peaks for several CPS during winter and spring matched documented spawning windows, underscoring the importance of fjords as seasonal spawning habitat. This globally transferable approach can supplement local stewardship initiatives and long-term monitoring efforts, contributing to more effective marine conservation area design and management. Les fjords sont des estuaires en eau profonde qui abritent un large éventail d'habitats marins pour les poissons pélagiques. Pourtant, les lacunes dans les connaissances sur la distribution des poissons et l'utilisation de l'habitat limitent la mise en œuvre de stratégies efficaces de conservation marine dans les fjords. En utilisant les fjords de la Colombie-Britannique comme étude de cas, nous avons démontré que le métabarcodage de l'ADNe combiné aux caractéristiques des espèces peut améliorer la détection et la surveillance des espèces prioritaires pour la conservation (CPS) dans le cadre de plans de conservation systématiques. Des échantillons ont été prélevés à quatre moments : fin janvier/février (hiver), mai (printemps), août (été) et novembre (automne) 2023 dans les cinq fjords de la Colombie-Britannique : Indian Arm (səl ̓ilwət in hən̓q̓əmin̓əmde), Howe Sound (Átl'a7tsem à Squamish), Toba Inlet (yṣkamṣamn à Homalco), Bute Inlet et Rivers Inlet (Wánu-vaxsiwa à Oowekyala). Trois à cinq stations ont été échantillonnées dans chaque fjord et des échantillons discrets d'ADN environnemental (ADNe) de 2 L ont été prélevés à l'aide de bouteilles Niskin à la surface, à 10 m sous la surface, à 100 m sous la surface et à 10 m au-dessus du fond. Les données de température et de salinité ont également été collectées à chaque instant à l'aide d'un CTD. À partir des échantillons d'ADNe, la communauté de poissons a été cartographiée à l'aide d'un métabarcoding ciblant une petite région du gène de l'ARNr 12S mitochondrial à l'aide du jeu d'amorces universelles miFISH-U. Des bibliothèques de séquençage indépendantes ont été préparées pour chacun des quatre ensembles de données saisonniers, et chacun a été séquencé à l'aide de la chimie Illumina MiSeq V3 au Hakai Genome Lab sur l'île Quadra, en Colombie-Britannique. Les profils d'ADNe spatiotemporels ont confirmé la présence d'eulakane (Thaleichthys pacificus) en voie de disparition dans trois fjords et ont identifié les habitats des fjords profonds comme des priorités de conservation pour les espèces mésopélagiques, notamment les élasmobranches sensibles et les migrateurs verticaux quotidiens tels que la lampfish nordique (Stenobrachius leucopsarus). Les pics d'ADNe de plusieurs CPS en hiver et au printemps correspondaient aux périodes de frai documentées, soulignant l'importance des fjords Les morues comme habitat de frai saisonnier. Cette approche transférable à l'échelle mondiale peut compléter les initiatives locales d'intendance et les efforts de surveillance à long terme, contribuant ainsi à une conception et à une gestion plus efficaces des aires marines de conservation. oceans true -128.343,51.8489 -126.3333,51.815 -122.6652,49.5126 -123.9831,49.2554 -128.343,51.8489 true ca.cioos 1 {'en': 'Coastal British Columbia Fjords', 'fr': 'Fjords côtiers de la Colombie-Britannique', 'translations': {'fr': {'message': "text translated using the Amazon translate service / texte traduit à l'aide du service de traduction Amazon", 'verified': False}}} 0.0 595.0 5829 EPSG 2012-08-10 EPSG::5829 - Instantaneous Water Level height - Height relative to instantaneous water level uncorrected for tide. Not specific to any location or epoch. vertical 2023-01-31T20:00:00.000Z 2023-11-23T20:00:00.000Z asNeeded Generated from https://hakaiinstitute.github.io/hakai-metadata-entry-form#/en/hakai/jnK2pbrIzbMBHpSiUOi8XDUeev93/-Ow-ZgW1jkC5-WyrtlLV Oceans Government of Canada Core Subject Thesaurus 2016-10-13 N/A Fjord Rivers Inlet Bute Inlet Toba Inlet Howe Sound Indian Arm 12S metabarcoding eDNA Environmental DNA Fish eDNA default fishAbundanceAndDistribution subSurfaceTemperature subSurfaceSalinity Abondance et distribution de poissons Température sous la surface Salinité sous la surface eov Genomics Oceanography project Creative Commons Attribution 4.0 Attribution CC-BY-4.0 https://creativecommons.org/licenses/by/4.0 WWW:LINK Paper: eDNA Metabarcoding Identifies Fish Conservation Priorities in Deep-water Fjords of British Columbia, Canada Article : Le métabarcodage eDNA identifie les priorités en matière de conservation des poissons dans les fjords en eau profonde de la Colombie-Britannique, au Canada DOI https://doi.org/10.1111/con4.70058 Fjord eDNA Biodiversity Shiny App Application Fjord DNA Biodiversity Shiny URL https://fjedna.shinyapps.io/census-app1/ University of British Columbia Vancouver Canada https://www.ubc.ca/ WWW:LINK https://ror.org/03rmrcq20 Jacquemot, Loïc loic.jacquemot42@gmail.com https://orcid.org/0000-0001-7509-840X Hakai Institute Campbell River Canada data@hakai.org https://www.hakai.org/ WWW:LINK https://ror.org/02pry0c91 https://www.ncbi.nlm.nih.gov/bioproject/?term=1289220 WWW:LINK NCBI BioProject: PRJNA1289220 Bioprojet NCBI : PRJNA1289220 Raw sequence data published to NCBI SRA Données de séquence brutes publiées sur NCBI SRA https://github.com/HakaiInstitute/Fjords_conservation WWW:LINK Fjords Conservation Github Repository Référentiel Github pour la conservation des fjords Processed sequence data repository Référentiel de données de séquences traitées asNeeded